About applications II

           Q1:    Could ozone use to treat airborne bacteria?

           A1:    Yes. The following table shows CT value relates to various airborne bacteria.                    

Microorganisms

Temp.

Relative humidity

CT (ppm x min)

Survival (%)

Reference

  Aspergillus fumigatus

--

--

0.3-0.9 x 240      

8

  Dyas et al. (1983)

  Aspergillus niger

--

35-76

0.1 x 240 = 24      

84

  Hibben and Stotzky (1969)

  Bacillus cereus

--

95

3 x 60 = 180      

0.013

  Ishizaki et al. (1986)

  Bacillus subtilis

23

51

0.2 x 720 = 144      

Nd*

  Klanova and Lajcikova. (2006)

  Candida albicans

23

51

0.2 x 720 = 144      

Nd*

  Klanova and Lajcikova. (2006)

  Escherichia coli

23

51

0.2 x 720 = 144      

Nd*

  Klanova and Lajcikova. (2006)

  Fusarium oxysporum

--

35-75

0.1 x 240 = 24      

2

  Hibben and Stotzky (1969)

  Penicillium chrysogenum

--

90

3-9 x 1380      

0.1

  Foarde et al. (1997)

  Proteus

--

--

0.3-0.9 x 240      

0.9

  Dyas et al. (1983)

  Pseudomonas aeruginosa

23

51

0.2 x 720 = 144      

Nd*

  Klanova and Lajcikova. (2006)

--

--

0.3-0.9 x 240      

31

  Dyas et al. (1983)

  Rhizopus stolonifer

--

35-77

0.1 x 240 = 24      

43

  Hibben and Stotzky (1969)

  Serratia spp.

--

--

0.3-0.9 x 240      

3.2

  Dyas et al. (1983)

  Staphylococcus aureus

23

51

0.2 x 720 = 144      

Nd*

  Klanova and Lajcikova. (2006)

--

--

0.3-0.9 x 240      

0.5

  Dyas et al. (1983)

  Streptococcus epidermis

--

--

0.6 x 4 = 2.4      

0.6

  Kowalski et al. (1998)

  Streptococcus salivarius

--

60-75

0.6 x 100 = 60      

2

  Elford and van de Eude (1942)

  PX174 (virus)

--

--

0.4 x 8 = 3.2      

0.01

  Kowalski et al. (1998)

 

                 The conclusions of Mr.  David P. Chynoweth's presentation "Influence of Ozone Air Treatment on Growth of Bacteria and 

                 Fungi in Air Conditioning Systems" indicates the following results:

                 I. Concentration of 0.0044 mg/l, duration of 30 min and frequency of 2 times per day prevented visible accumulation of microbial

                     biofilm in an A/C unit over a period of six month.

                 II. Concentration of 0.0044 mg/l, duration of 120 min and frequency of 2 times per day resulted in total inhibition of Serratia  and

                      partial inhibition of Aspergillus .

                 III. Concentration of 0.018 mg/l, duration of 15 min and frequency of 24 times per day resulted in total inhibition of Serratia and

                       Aspergillus.

                 IV. Concentration of 0.018 mg/l, duration of 15 min and frequency of 6 times per day resulted in total inhibition of natural 

                      airborne organisms.

                 (Mr.  David P. Chynoweth: Agricultural and Biological Engineering Dept. University of Florida, Gainesville, FL)

                                

            Reference

               Dyas, A., B. J. Boughton and B. C. Das.  1983.  Ozone killing action against bacterial and fungal species; microbiological testing of

                       a domestic ozone generator.  J. Clin. Path. 36: 1102-1104.

               Elford, W. J. and J. van de Eude.  1942.  An investigation of the merits of ozone as an aerial disinfectant.  J. Hygiene 42: 240-265.

               Foarde, K. K., D. W. VanOsdell and R. S. Steiber.  1997.  Investigation of gas-phase ozone as a potential biocide.  Appl. Occup. 

                       Environ. Hyg. 12: 535-541.

               Hibben, C. R. and G. Stotzky.  1969.  Effects of ozone on thegermination of fungus spores.  Can. J. Microbiol. 15: 1187-1196.

               Ishizaki, K., N. Shinriki and H. Matsuyama.  1986.  Inactivation of Bacillus spores by gaseous ozone.  J. Appl. Act. 60: 67-72.

               Klanova K. and A. Lajcikova.  2006.  Use of ozone to reduce Bacteria and Moulds in the air and on surface.  Indoor Built Environ 

                       15-1: 81-84.

               Kowalski, W. J., W. P. Bahnfleth and T. S. Whittam.  1998.  Bactericidal effects of high airborne ozone concentrations on Escherichia 

                       coli and Staphylococcus aureus.  Ozone Science and Engineering 20: 205-221.